2.5

Bioreactor

1. T-75 flask.

2. 1-Layer cell stack.

3. 125–212 μM polystyrene microcarriers.

Fig. 1 Assemble the dip tube/perfusion line. This allows for the continuous

perfusion of cell culture medium, while retaining the cells in the bioreactor

vessel during the expansion process

Fig. 2 Assemble the media feed line. This allows for the introduction of cell

culture medium during the expansion process

Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors

41